WebAug 11, 2010 · ESP has been described as a thermal unstable protein (Matusheski et al. 2004) and is dependent on Fe 2+ for activity (MacLeod and Rossiter 1985; Williams et … WebHeating decreases epithiospecifier protein activity and increases sulforaphane formation in broccoli. Nathan V. Matusheski;John A. Juvik;Elizabeth H. Jeffery. ... Advances in Botanical Research (2010) 295 Citations Epithiospecifier Protein from Broccoli (Brassica oleracea L. ssp. italica) Inhibits Formation of the Anticancer Agent Sulforaphane ...
Molecules Free Full-Text Molecular Modeling of Epithiospecifier …
WebOct 16, 2013 · The epithiospecifier modifier 1 (ESM1) gene in Arabidopsis encodes a protein shown to inhibit function of ESP, leading to increased isothiocyanate production from GS hydrolysis . Methyl jasmonate (MeJA), a plant signal transduction compound associated with herbivore defense, can act as an elicitor to enhance GS biosynthesis . WebJan 7, 2012 · The expression of epithiospecifier protein (ESP) was assessed at the transcript level by qPCR and at the protein level by Western blot in the two A. thaliana ecotypes Col-0 and Mt-0. ESP transcript (Fig. 2 ) and ESP protein levels (Fig. 3 ) were much higher in Mt-0 than in Col-0, which is consistent with high ESP activity in Mt-0 but … now she\u0027s gone gone gone
Identification and Characterization of Three …
WebApr 4, 2024 · However, the existence of the epithiospecifier protein could direct the hydrolysis of glucosinolates toward sulforaphane nitrile formation instead of sulforaphane. Therefore, the study aimed on improving the yielding of sulforaphane in broccoli sprouts with a new method of the united hydrolysis of cruciferous sprouts. WebMay 17, 2024 · Sulforaphane is a chemical compound rich in sulfur present in cruciferous vegetables such as Brussels sprouts, cauliflower, cabbage, arugula, broccoli, and broccoli sprouts. It is thought to help prevent cancers and combat free radicals as an antioxidant. 1. Other research points to sulforaphane as an anti-inflammatory and DNA protector. WebBiochemical characterization of heterologous expressed BoESP isoforms revealed different substrate specificities towards seven glucosinolates: all isoforms showed … now she\u0027s gone and i\u0027m on my own