Incipient cytorrhysis assay

WebApr 11, 2014 · We applied incipient cytorrhysis to investigate the appressorial turgor pressure of P. pachyrhizi. Mature appressoria were … Webincipient cytorrhysis assays indicate that turgor pressure begins to be generated in the appressorium from 16 h (Fig. 1) and penetration of the rice cuticle can occur from 24 h …

MoPex19, which Is Essential for Maintenance of Peroxisomal …

WebFeb 9, 2012 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–5 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspension (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature. WebNov 16, 2024 · An incipient cytorrhysis assay was carried out by allowing appressoria to form in water on borosilicate cover slips for 24 hr, after which the water was replaced with a range of aqueous glycerol ranging from 0.25 to 2.5M and, after 30 min, the frequency of … ray white event https://fairysparklecleaning.com

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WebApr 16, 2024 · To determine whether the appressorial turgor pressure was altered in ∆mopex13 and ∆mopex14, we performed an incipient cytorrhysis assay ). The collapse rates of the appressoria of ∆mopex14 treated with glycerol were increased compared with the wild type, suggesting a reduction of the turgor generation in ∆mopex14 appressoria. WebJan 14, 2014 · The incipient-cytorrhysis assay was performed to measure appressorial turgor. Conidia in 20 μl droplets (2×10 5 /ml) were allowed to form appressoria on plastic coverslips for 48 h. Water surrounding the conidia was removed carefully and replaced with 20 μl 0.5–4.0 M glycerol. The number of collapsed appressoria was counted after 10 min. WebJan 26, 2024 · To investigate whether the reduced pathogenicity of ΔMopcs60 was associated with appressorium-mediated penetration, we first determined the rate of appressorium collapse in the presence of various hyperosmotic concentrations of glycerol by performing an incipient cytorrhysis assay. simply southern pattern

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Incipient cytorrhysis assay

MoPex19, which Is Essential for Maintenance of Peroxisomal …

WebDec 20, 2011 · The appressorial turgor pressure was determined using the incipient cytorrhysis assay, as reported previously ( Howard et al. 1991 ). Each assay was completely independent and replicated at least three times. Plant infection assays WebFeb 18, 2024 · Considering that the germinating and appressorial formation were normal in the Mohat1 mutant (Table S1), we measured the internal concentration of solute and the turgor pressure within appressoria by the incipient cytorrhysis (cell collapse) assay using 1–4 molar of glycerol [ 45 ].

Incipient cytorrhysis assay

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WebJan 15, 2003 · Appressorium turgor can be measured using an incipient cytorrhysis assay which uses hyperosmotic concentrations of a solute to collapse appressoria, thereby … WebAug 4, 2014 · (A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying …

WebNov 2, 2015 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5-molar concentration of glycerol solution, as previously described (Foster et al ., 2003 ). Visualization of glycogen and lipid during appressorium development Appressorium formation was induced on the coverslips (Fisher Scientific). WebJan 26, 2016 · Appressorium turgor was measured by incipient cytorrhysis assay using a 2- to 5 M glycerol solution as previously described (Foster et al. 2003). The onion penetration assay was performed as previously described (Wang et al. 2013). Onion epidermal strips were isolated and 40 μl of conidial suspension was inoculated onto the adaxial surface.

WebDec 20, 2024 · While a confocal microscope was used for investigation of subcellular localizations, light microscopy using Olympus DP80 (Tokyo, Japan) was utilized for conidiophores formation assay, cytorryhsis incipient assay, invasive hyphae development and appressorium penetration and glycogen degradation. 2.5. WebJan 26, 2024 · To investigate whether the reduced pathogenicity of ΔMopcs60 was associated with appressorium-mediated penetration, we first determined the rate of …

WebJul 5, 2016 · The appressorium turgor was measured using an incipient cytorrhysis (cell collapse) assay and a 1–4 m glycerol solution (Howard et al., 1991). Droplets (20 µL) of the conidial suspensions (5 × 10 4 spores/mL) were placed on plastic coverslips and incubated in a humid chamber for 24 h at room temperature.

WebIncipient cytorrhysis determined the turgor pressure in appressoria of P. pachyrhizi to be equivalent to 5.13 MPa. MZM data revealed that osmotically active sugar alcohols only … ray white fairfield nswWebIn an incipient cytorrhysis (cell collapse) assay using a 1–4 molar concentration of glycerol solution, appressoria of the ΔMomip11 mutant showed increased collapse rates in … simply southern peach sherpa pulloversimply southern peacemaker shirtWebApr 6, 2007 · Appressorial turgor was estimated using incipient cytorrhysis (cell collapse) assays ( 6 ). Conidia in 20-μl droplets (1 × 10 6 conidia/ml) were induced to form appressoria on plastic microscope coverslips incubated in a humid chamber for 24 h or 48 h. simply southern pearl necklaceWebAug 3, 2024 · Glycogen staining and cytorrhysis assays Appressorium turgor was measured using an incipient cytorrhysis assay. Twenty microlitres of the conidial suspension (5 × 10 4 spores/ml) was placed on hydrophobic coverslips and incubated in a humid chamber for 24 h at room temperature. simply southern paris kyWeb(A) To measure appressorial turgor pressure, incipient cytorrhysis assay was performed on induced appressoria at 48 hpi with glycerol solutions of varying concentrations (1-4 M). simply southern peach pulloverWebJul 1, 2024 · Appressorium turgor was measured following the incipient cytorrhysis (cell collapse) assays ( Howard et al., 1991 ). The plastic coverslips were prepared to incubate droplets (20 μl) of conidial suspension (1 × 10 5 conidia/mL). The setup was placed in a humid chamber for 24 h. simply southern peanut butter and jelly shirt