WebPX458 (Search Vector Database) Total vector size (bp) 9300 Vector type Mammalian Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Ampicillin, 100 μg/mL … pSpCas9(BB)-2A-GFP (PX458) Sequences (11) Addgene Sequences: Full (1) Parti… Image: Illustrated plasmid map in PNG format GenBank File: Plasmid sequence a… Plasmid pSpCas9n(BB)-2A-Puro (PX462) from Dr. Feng Zhang's lab contains the i… Plasmid pSpCas9n(BB)-2A-GFP (PX461) from Dr. Feng Zhang's lab contains the in… WebHeLa cells transfected with PX458 (pSpCas9(BB)-2A-GFP) (green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min. The cells were then incubated with GenCRISPRᵀᴹ SpCas9 Antibody (14B6) (GenScript, A01936-40) at 2 μg/ml, 1 μg/ml and mouse IgG at 2 μg/ml, 1 μg/ml at room temperature for 2h, followed by a further ...
pSpCas9(BB)-2A-GFP (PX458) - CRISPR Human - Deletion AURKB
WebImmunofluorescence staining of GenCRISPR™ SpCas9 Antibody (4A1) (GenScript, A01935-40) with SpCas9 in Hela cells. HeLa cells transfected with PX458 (pSpCas9 (BB)-2A-GFP) (green) were fixed with 4% Poly-Formaldehyde (5min) and then blocked in 3% BSA 30min. WebP Spcas9 Bb 2a Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... Spcas9 Bb 2a Gfp Px458, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS ... ce kaiser
SpCas9 Monoclonal Antibody (4A1) (A01935-40) - Thermo Fisher Scientific
WebThe selected sgRNA sequences are listed in . The sgRNA and SpCas9 expression cassette from PX458 (pSpCas9 (BB)-2A-GFP) or PX459 (pSpCas9 (BB)-2A-Puro) (both plasmids … WebJan 25, 2024 · pSpCas9(BB)-2A-GFP (PX458) (Addgene #48138) and pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988) were used as vectors to integrate sequences coding for full-length gRNAs carrying either the ... WebSteroid-resistant nephrotic syndrome (SRNS) is a frequent cause of CKD. The discovery of monogenic causes of SRNS has revealed specific pathogenetic pathways, but these … ce konformität